Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a . Schematic of the tagged ENaCγ subunit produced from the ENaCγ-VF allele, which includes a C-terminal mVenus, 3C protease site, and 3xFLAG tag. b . Representative immunofluorescence image showing co-localization of mVenus and ENaCγ in kidney tissue. mVenus signal was detected using a FITC-conjugated anti-GFP primary antibody (goat, Rockland, 1:100) and Alexa Fluor 488 donkey anti-goat secondary antibody. Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody. DAPI is 4’,6-diamidino-2-phenylindole . c . Close-up view highlighting localization of tagged ENaCγ.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Produced, Immunofluorescence

Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a. Cryo-EM map of human ENaC in complex with two Fabs, 10D4 and 7B1, shown in side and top-down views. In the top-down view, a red box highlights the interaction site between 7B1 and the α subunit. b. Sequence alignment of the experimentally determined 7B1 epitope region in human and mouse ENaCα subunits, revealing high conservation and supporting the potential for cross-reactivity with mouse ENaC. c . Schematic representation of the 7B1-mScarlet Fab construct used to detect the ENaC α subunit. The variable regions of the 7B1 heavy and light chains were expressed as a Fab fragment in HEK293 cells, with mScarlet fused to the C-terminus of the heavy chain. d . FSEC trace monitoring mVenus fluorescence from lung lysates of ENaCγ-VF mice, showing the elution profile of γ-containing ENaC complexes. Dotted lines indicate the elution range for mVenus-tagged complexes (11-16 mL). e . FSEC trace from the same sample following 7B1-mScarlet fluorescence, revealing the elution profile of α-containing ENaC complexes. Dashed lines show that the α-associated signal is restricted to a narrower elution range (11-13 mL), suggesting that α-γ co-assemblies represent a more defined subset of the total γ-containing complexes. f . Overlay of the FSEC traces shown in panels d and e , plotted on the same axes to facilitate direct comparison of elution profiles and peak positions. The inset shows the full normalized traces for each condition. The main panel displays a magnified view of the gray-shaded region indicated in the inset, highlighting the relative alignment of the major peaks.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Cryo-EM Sample Prep, Sequencing, Construct, Fluorescence, Comparison